The Union branch raised the funds from a raffle at the Anglesey County Show in August.
The Wales Air Ambulance Charity is funded by the people of Wales, and they operate some of the most advanced air ambulances in the UK, saving precious time and lives.
FUW Anglesey county chair, William Hughes, said: "They are there for the people of Wales whenever and wherever they need them, and I'm delighted that we could present them with this contribution and support this very worthy cause."
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The Wales Air Ambulance requires £11.2 million every year to keep the helicopters in the air and rapid response vehicles on the road across Wales.
They offer advanced critical care and are often described as a ‘Flying ED'.
The on-board consultants and critical care practitioners are highly skilled and carry some of the most pioneering medical equipment in the world.
They can deliver blood transfusions, administer anaesthesia and undertake emergency operations at the scene of the incident, before flying the patient directly to specialist care.
The Charity has completed over 46,000 missions and is on standby 24 hours a day, 365 days a year.
The meeting was held at the YFC Centre, Anglesey Agricultural Show Site.
See also: Farming siblings leave £1.3 million to Wales Air Ambulance